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Imaging and probing cell mechanical properties with the atomic force microscope.

Authors
Type
Published Article
Journal
Methods in Molecular Biology
1064-3745
Publication Date
Volume
319
Pages
331–361
Identifiers
PMID: 16719364
Source
Medline

Abstract

This chapter describes the use of the atomic force microscope (AFM) to probe and map out regional variations in apparent elastic properties of living cells. The importance of mechanics in the field of cell biology is becoming more widely appreciated, and the AFM has unique advantages for cell mechanics applications. However, care must be taken in the acquisition, analysis, Band interpretation of AFM indentation data. To help make this powerful technique accessible to a broad range of investigators, detailed procedures are provided for all stages of the AFM experiment from sample preparation through data analysis and visualization.

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