Affordable Access

deepdyve-link
Publisher Website

Imaging Biotin Trafficking In Vivo with Positron Emission Tomography

Authors
  • Bongarzone, Salvatore
  • Sementa, Teresa
  • Dunn, Joel
  • Bordoloi, Jayanta
  • Sunassee, Kavitha
  • Blower, Philip J.
  • Gee, Antony
Type
Published Article
Journal
Journal of Medicinal Chemistry
Publisher
American Chemical Society
Publication Date
Jul 13, 2020
Volume
63
Issue
15
Pages
8265–8275
Identifiers
DOI: 10.1021/acs.jmedchem.0c00494
PMID: 32658479
PMCID: PMC7445742
Source
PubMed Central
License
Unknown

Abstract

The water-soluble vitamin biotin is essential for cellular growth, development, and well-being, but its absorption, distribution, metabolism, and excretion are poorly understood. This paper describes the radiolabeling of biotin with the positron emission tomography (PET) radionuclide carbon-11 ([11C]biotin) to enable the quantitative study of biotin trafficking in vivo. We show that intravenously administered [11C]biotin is quickly distributed to the liver, kidneys, retina, heart, and brain in rodents—consistent with the known expression of the biotin transporter—and there is a surprising accumulation in the brown adipose tissue (BAT). Orally administered [11C]biotin was rapidly absorbed in the small intestine and swiftly distributed to the same organs. Preadministration of nonradioactive biotin inhibited organ uptake and increased excretion. [11C]Biotin PET imaging therefore provides a dynamic in vivo map of transporter-mediated biotin trafficking in healthy rodents. This technique will enable the exploration of biotin trafficking in humans and its use as a research tool for diagnostic imaging of obesity/diabetes, bacterial infection, and cancer.

Report this publication

Statistics

Seen <100 times