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IgE and IgG binding of peptides expressed from fragments of cDNA encoding the major house dust mite allergen Der p I.

Authors
  • Greene, W K
  • Cyster, J G
  • Chua, K Y
  • O'Brien, R M
  • Thomas, W R
Type
Published Article
Journal
Journal of immunology (Baltimore, Md. : 1950)
Publication Date
Dec 01, 1991
Volume
147
Issue
11
Pages
3768–3773
Identifiers
PMID: 1940366
Source
Medline
License
Unknown

Abstract

Large peptides expressed from cDNA fragments of a clone encoding the mite allergen Der p I were able to bind IgE and IgG in sera from allergic individuals. The binding was found for peptides from sequences throughout the molecule, with at least five regions, comprising residues 1-56, 53-99, 98-140, 166-194, and 188-222. The only limitation was that more than 30 amino acid residues were required for consistent binding. Each of seven sera examined showed a different profile of antibody binding to the peptides. For the most part the pattern of IgE and IgG binding to the peptides for each serum was similar, demonstrating a concordant repertoire. In 5/7 sera, however, IgG bound to some peptides which had little or no IgE binding activity, thus showing more diverse specificities. It is suggested that some divergence of repertoire can develop during the maturation of the B cell response.

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