Affordable Access

Access to the full text

IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo

Authors
  • Vivar, Omar I.
  • Masi, Giulia
  • Carpier, Jean-Marie
  • Magalhaes, Joao G.
  • Galgano, Donatella
  • Pazour, Gregory J.
  • Amigorena, Sebastian
  • Claire Hivroz
  • Baldari, Cosima T.
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Jul 01, 2016
Volume
113
Issue
2
Pages
91–386
Identifiers
DOI: 10.1073/pnas.1513601113
PMID: 26715756
PMCID: PMC4720349
Source
USPC - SET - SVS
License
Green

Abstract

Biogenesis of the immune synapse at the interface between antigen-presenting cells and T cells assembles and organizes a large number of membrane proteins required for effective signaling through the T-cell receptor. We showed previously that the intraflagellar transport protein 20 (IFT20), a component of the intraflagellar transport system, controls polarized traffic during immune synapse assembly. To investigate the role of IFT20 in primary CD4+ T cells in vitro and in vivo, we generated mice bearing a conditional defect of IFT20 expression in T cells. We show that in the absence of IFT20, although cell spreading and the polarization of the centrosome were unaffected, T-cell receptor (TCR)-mediated signaling and recruitment of the signaling adaptor LAT (linker for activation of T cells) at the immune synapse were reduced. As a consequence, CD4+ T-cell activation and proliferation were also defective. In vivo, conditional IFT20-deficient mice failed to mount effective antigen-specific T-cell responses, and their T cells failed to induce colitis after adoptive transfer to Rag−/− mice. IFT20 is therefore required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes and for effective T-cell responses in vivo.

Report this publication

Statistics

Seen <100 times