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IFN-mediated induction of MHC antigen expression on human keratinocytes and its influence on in vitro alloimmune responses.

Authors
  • Niederwieser, D
  • Auböck, J
  • Troppmair, J
  • Herold, M
  • Schuler, G
  • Boeck, G
  • Lotz, J
  • Fritsch, P
  • Huber, C
Type
Published Article
Journal
Journal of immunology (Baltimore, Md. : 1950)
Publication Date
Apr 15, 1988
Volume
140
Issue
8
Pages
2556–2564
Identifiers
PMID: 2451691
Source
Medline
License
Unknown

Abstract

The MHC Ag expression on the surface of keratinocytes is altered after treatment with IFN. IFN-gamma induces, as expected, a strong increase in class I MHC Ag expression as well as de novo expression of class II MHC Ag, whereas IFN-alpha 2 only slightly increases class I MHC Ag and does not induce keratinocytes to express class II MHC Ag. We used untreated and IFN-pretreated keratinocytes as stimulators and also as targets to study whether IFN-induced MHC Ag changes would alter the immunogenicity of keratinocytes in alloimmune responses. It was found that class II MHC Ag-carrying keratinocytes were unable to induce the proliferation of resting lymphocytes, but did stimulate T blasts. Untreated keratinocytes were virtually resistant to the lysis by classical CTL but became susceptible after exposure to IFN-gamma, but not IFN-alpha 2 at physiologic doses. These data demonstrate mechanisms by which the release of IFN-gamma might contribute to the development of disease such as the graft vs host disease.

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