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Identification of yeasts by hydrolysis of amides.

Authors
  • Mira-Gutiérrez, J
  • Garcia-Martos, P
  • Mira-Gordillo, A J
Type
Published Article
Journal
Mycoses
Publication Date
Jan 01, 1995
Volume
38
Issue
3-4
Pages
101–106
Identifiers
PMID: 7477083
Source
Medline
License
Unknown

Abstract

We analysed the hydrolysis of 23 amides by 500 yeast and yeast-like strains isolated from clinical specimens, identified to species level by conventional methods, in order to verify the validity of this method of species identification. The results show that 10 of these amides (acetamide, acrylamide, alaninamide, formamide, glycinamide, propionamide, urea, thioacetamide, thiourea and valeramide) are sufficient to differentiate seven genera and 19 species, with an occasional requirement for three additional tests: cycloheximide susceptibility, surface film formation on liquid medium and ascospore formation. The study of the amidase activity in yeasts and yeast-like fungi seems to be a promising method of identifying strains isolated from clinical samples.

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