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Identification of two families of satellite-like repetitive DNA sequences from the zebrafish (Brachydanio rerio).

Authors
  • Ekker, M
  • Fritz, A
  • Westerfield, M
Type
Published Article
Journal
Genomics
Publication Date
Aug 01, 1992
Volume
13
Issue
4
Pages
1169–1173
Identifiers
PMID: 1339388
Source
Medline
License
Unknown

Abstract

To further our understanding of the structure and organization of the zebrafish genome, we have undertaken the analysis of highly and middle-repetitive DNA sequences. We have cloned and sequenced two families of tandemly repeated DNA fragments. The monomer units of the Type I satellite-like sequence are 186 bp long, A+T-rich (65%), and exhibit a high degree of sequence conservation. The Type I satellite-like sequence constitutes 8% of the zebrafish genome, or approximately 8 x 10(5) copies per haploid genome. Southern analysis of genomic DNA, digested with several restriction endonucleases, shows a ladder of hybridizing bands, consistent with a tandem array, and suggests longer range periodic variations in the sequence of the tandem repeats. The Type II satellite has a monomer length of 165 bp, is also A+T-rich (68%), and constitutes 0.2% of the zebrafish genome (22,000 copies per haploid genome). Southern analysis reveals a complex pattern rather than a ladder of regularly spaced hybridizing bands.

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