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Identification of transcripts encoding a parathyroid hormone-like peptide in messenger RNAs from a variety of human and animal tumors associated with humoral hypercalcemia of malignancy.

Authors
  • Ikeda, K1
  • Mangin, M
  • Dreyer, B E
  • Webb, A C
  • Posillico, J T
  • Stewart, A F
  • Bander, N H
  • Weir, E C
  • Insogna, K L
  • Broadus, A E
  • 1 Department of Medicine, Yale University, New Haven, Connecticut 06511.
Type
Published Article
Journal
The Journal of clinical investigation
Publication Date
June 1988
Volume
81
Issue
6
Pages
2010–2014
Identifiers
PMID: 2454953
Source
Medline
License
Unknown

Abstract

The syndrome of humoral hypercalcemia of malignancy (HHM) appears to be mediated in many instances by a parathyroid hormone-like peptide, which has recently been purified, sequenced, and cloned. Using a probe representing the coding region of the human PTH-like peptide, we examined by Northern analysis poly (A)+ RNA from a variety of human and animal tumors associated with HHM. Hybridizing transcripts were identified in mRNA from each of 12 human and each of four animal HHM-associated tumors, with a complex hybridization pattern observed in the human mRNAs and a relatively simple pattern observed in the animal mRNAs. Poly (A)+ RNA prepared from tumors of similar histological types unassociated with HHM failed to hybridize with the probe. Messenger RNA-dependent biological activity from the animal tumors was entirely eliminated in a hybridization-arrest experiment using a complementary oligonucleotide spanning the region of homology between human PTH and the PTH-like peptide. These findings indicate that the PTH-like peptide is associated with the syndrome of HHM in a wide spectrum of tumor types from a variety of mammalian species and that the PTH-like sequence in the proximal amino terminus of the peptide is highly conserved.

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