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Identification, structural characterisation and expression analysis of a defensin gene from the tiger beetle Calomera littoralis (Coleoptera: Cicindelidae).

Authors
  • Rodríguez-García, María Juliana1
  • García-Reina, Andrés2
  • Machado, Vilmar2
  • Galián, José2
  • 1 University of Murcia, Department of Zoology and Physical Anthropology, Faculty of Veterinary, Campus Mare Nostrum, E-30100 Murcia, Spain. Electronic address: [email protected] , (Spain)
  • 2 University of Murcia, Department of Zoology and Physical Anthropology, Faculty of Veterinary, Campus Mare Nostrum, E-30100 Murcia, Spain. , (Spain)
Type
Published Article
Journal
Gene
Publication Date
Sep 01, 2016
Volume
589
Issue
1
Pages
56–62
Identifiers
DOI: 10.1016/j.gene.2016.05.030
PMID: 27210512
Source
Medline
Keywords
License
Unknown

Abstract

In this study, a defensin gene (Clit-Def) has been characterised in the tiger beetle Calomera littoralis for the first time. Bioinformatic analysis showed that the gene has an open reading frame of 246bp that contains a 46 amino acid mature peptide. The phylogenetic analysis showed a high variability in the coleopteran defensins analysed. The Clit-Def mature peptide has the features to be involved in the antimicrobial function: a predicted cationic isoelectric point of 8.94, six cysteine residues that form three disulfide bonds, and the typical cysteine-stabilized α-helix β-sheet (CSαβ) structural fold. Real time quantitative PCR analysis showed that Clit-Def was upregulated in the different body parts analysed after infection with lipopolysaccharides of Escherichia coli, and also indicated that has an expression peak at 12h post infection. The expression patterns of Clit-Def suggest that this gene plays important roles in the humoral system in the adephagan beetle Calomera littoralis.

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