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Identification of the proteome of the midgut of silkworm, Bombyx mori L., by multidimensional liquid chromatography (MDLC) LTQ-Orbitrap MS.

Authors
  • Yao, Hui-Peng
  • Xiang, Xing-Wei
  • Chen, Lin
  • Guo, Ai-Qin
  • He, Fang-Qing
  • Lan, Li-Pan
  • Lu, Xing-Meng
  • Wu, Xiao-Feng
Type
Published Article
Journal
Bioscience Reports
Publisher
Portland Press
Publication Date
Dec 01, 2009
Volume
29
Issue
6
Pages
363–373
Identifiers
DOI: 10.1042/BSR20080144
PMID: 19007334
Source
Medline
License
Unknown

Abstract

The midgut is the digestive apparatus of the silkworm and its proteome was studied by using nano-LC (liquid chromatography) electrospray ionization MS/MS (tandem MS). MS data were analysed by using X!Tandem searching software using different parameters and validated by using the Poisson model. A total of 90 proteins were identified and 79 proteins were described for the first time. Among the new proteins, (i) 22 proteins were closely related to the digestive function of the midgut, including 11 proteins of digestive enzymes secreted by the epithelium, eight proteins of intestine wall muscle and mechanical digestion and three proteins of peritrophic membrane that could prevent the epithelium from being mechanically rubbed; (ii) 44 proteins were involved in metabolism of substance and energy; and (iii) 11 proteins were associated with signal transduction, substance transport and cell skeleton.

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