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Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach

Authors
  • Buffoni, Leandro1
  • Garza-Cuartero, Laura2
  • Pérez-Caballero, Raúl1, 3
  • Zafra, Rafael1
  • Javier Martínez-Moreno, F.1
  • Molina-Hernández, Verónica1
  • Pérez, José1
  • Martínez-Moreno, Álvaro1
  • Mulcahy, Grace2, 4
  • 1 University of Córdoba, Campus de Rabanales, Ctra. Madrid-Cádiz, km 396, Córdoba, 14014, Spain , Córdoba (Spain)
  • 2 University College Dublin, Belfield, D4, Dublin, Ireland , Dublin (Ireland)
  • 3 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA , Bethesda (United States)
  • 4 Conway Institute, University College Dublin, Dublin, Ireland , Dublin (Ireland)
Type
Published Article
Journal
Parasites & Vectors
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jul 31, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13071-020-04260-6
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundFasciolosis is one of the most important parasitic diseases of livestock. The need for better control strategies gave rise to the identification of various vaccine candidates. The recombinant form of a member of the cysteine protease family, cathepsin L1 of Fasciola hepatica (FhCL1) has been a vaccine target for the past few decades since it has been shown to behave as an immunodominant antigen. However, when FhCL1 was used as vaccine, it has been observed to elicit significant protection in some trials, whereas no protection was provided in others.MethodsIn order to improve vaccine development strategy, we conducted a linear B-cell epitope mapping of FhCL1 in sheep vaccinated with FhCL1, FhHDM, FhLAP and FhPrx plus Montanide and with significant reduction of the fluke burden, sheep vaccinated with FhCL1, FhHDM, FhLAP and FhPrx plus aluminium hydroxide and with non-significant reduction of the fluke burden, and in unvaccinated-infected sheep.ResultsOur study showed that the pattern and dynamic of peptide recognition varied noticeably between both vaccinated groups, and that the regions 55–63 and 77–84, which are within the propeptide, and regions 102–114 and 265–273 of FhCL1 were specifically recognised only by vaccinated sheep with significant reduction of the fluke burden. In addition, these animals also showed significant production of specific IgG2, whereas none was observed in vaccinated-Aluminium hydroxide and in infected control animals.ConclusionsWe have identified 42 residues of FhCL1 that contributed to protective immunity against infection with F. hepatica in sheep. Our results provide indications in relation to key aspects of the immune response. Given the variable outcomes of vaccination trials conducted in ruminants to date, this study adds new insights to improve strategies of vaccine development.

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