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Identification of potential urine biomarkers in idiopathic parkinson's disease using NMR.

Authors
  • Kumari, Sadhana1
  • Kumaran, S Senthil2
  • Goyal, Vinay3
  • Sharma, Raj Kumar4
  • Sinha, Neeraj4
  • Dwivedi, S N5
  • Srivastava, Achal Kumar6
  • Jagannathan, N R7
  • 1 NMR and MRI Facility, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. , (India)
  • 2 NMR and MRI Facility, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. Electronic address: [email protected] , (India)
  • 3 Department of Neurology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India; Director Neurology, Medanta, The Medicity, Gurgaon, India. , (India)
  • 4 Centre of Biomedical Research, SGPGI Campus, Lucknow, India. , (India)
  • 5 Department of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. , (India)
  • 6 Department of Neurology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. , (India)
  • 7 NMR and MRI Facility, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India; Professor of Eminence of Radiology, Chettinad Academy of Research & Education, Kelambakkam, TN 603103, India. , (India)
Type
Published Article
Journal
Clinica chimica acta; international journal of clinical chemistry
Publication Date
Aug 10, 2020
Volume
510
Pages
442–449
Identifiers
DOI: 10.1016/j.cca.2020.08.005
PMID: 32791135
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Parkinson's disease (PD) is the most common neurodegenerative disease caused by the loss of dopamine chemicals resulting in urinary incontinence, gastrointestinal dysfunction, gait impairment and mitochondrial dysfunction. Study investigated urinary metabolic profiles of patients with idiopathic PD as compared to healthy controls (HC) to identify the potential biomarkers. Urine samples were collected from 100 PD subjects and 50 HC using standard protocol. Metabolomic analyses were performed using high resolution nuclear magnetic resonance (NMR) spectroscopy. The integral values of 17 significant metabolites were estimated and concentration values were calculated, which were subjected to univariate and multivariate statistical analysis. We found significantly increased levels of ornithine, phenylalanine, isoleucine, β-hydroxybutyrate, tyrosine and succinate in the urine of patients with PD in comparison with HC. These metabolites exhibited area under the curve greater than 0.60 on ROC curve analysis. We also observed a significant association between succinate concentration and UPDRS motor scores of PD. Metabolic pathway alterations were observed in aromatic amino acid metabolism, ketone bodies synthesis, branched chain amino acid metabolism and ornithine metabolism. Comprehensive metabolomic profiling revealed variations in urinary signatures associated with severity of idiopathic PD. This profiling relies on non-invasive sampling and is complementary to existing clinical modalities. Copyright © 2020 Elsevier B.V. All rights reserved.

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