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Identification of Planctomycetes with Order-, Genus-, and Strain-Specific 16S rRNA-Targeted Probes

Authors
  • Gade, D.1, 2
  • Schlesner, H.2
  • Glöckner, F. O.1
  • Amann, R.1
  • Pfeiffer, S.3
  • Thomm, M.2
  • 1 Max-Planck-Institut für Marine Mikrobiologie, Celsiusstr. 1, D-28359 Bremen, Germany
  • 2 Universität Kiel, Am Botanischen Garten 1-9, D-24118 Kiel, Institut für Allgemeine Mikrobiologie, Germany
  • 3 Biologiezentrum der Universität Kiel, Am Botanischen Garten 5, D-24098 Kiel, Zentrale Mikroskopie, Germany
Type
Published Article
Journal
Microbial Ecology
Publisher
Springer-Verlag
Publication Date
Mar 04, 2004
Volume
47
Issue
3
Pages
243–251
Identifiers
DOI: 10.1007/s00248-003-1016-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

A specific 16S rRNA-targeted oligonucleotide probe (PIR1223) for the genus Pirellula and a species-specific probe (RB454) for Pirellula sp. strain SH1 have been designed and optimized. Together with the already existing order-specific probe PLA886, the two newly designed probes were used to detect and identify planctomycetes, pirellulae, and close relatives of Pirellula sp. strain SH1 in different habitats. With the help of these probes for detection and identification, bacteria of the genus Pirellula were detected and cultivated from tissue of the Mediterranean sponge Aplysinaaerophoba and from the water column of the Kiel Fjord. An unexpected result was the close phylogenetic relationship of the isolate from the sponge and the brackish water habitat Kiel Fjord as revealed by DNA/DNA hybridization.

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