Affordable Access

Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland.

Authors
  • Adaszek, Łukasz
  • Górna, Marta
  • Krzysiak, Michał
  • Adaszek, Michał
  • Garbal, Marcin
  • Winiarczyk, Stanisław
Type
Published Article
Journal
Wiadomości parazytologiczne
Publication Date
Jan 01, 2011
Volume
57
Issue
1
Pages
21–26
Identifiers
PMID: 21634230
Source
Medline
License
Unknown

Abstract

The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.

Report this publication

Statistics

Seen <100 times