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Identification of rat lung – prominent genes by a parallel DNA microarray hybridization

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Abstract ral ss BioMed CentBMC Genomics Open AcceResearch article Identification of rat lung – prominent genes by a parallel DNA microarray hybridization Zhongming Chen, Jiwang Chen, Tingting Weng, Nili Jin and Lin Liu* Address: Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA Email: Zhongming Chen - [email protected]; Jiwang Chen - [email protected]; Tingting Weng - [email protected]; Nili Jin - [email protected]; Lin Liu* - [email protected] * Corresponding author Abstract Background: The comparison of organ transcriptomes is an important strategy for understanding gene functions. In the present study, we attempted to identify lung-prominent genes by comparing the normal transcriptomes of rat lung, heart, kidney, liver, spleen, and brain. To increase the efficiency and reproducibility, we first developed a novel parallel hybridization system, in which 6 samples could be hybridized onto a single slide at the same time. Results: We identified the genes prominently expressed in the lung (147) or co-expressed in lung- heart (23), lung-liver (37), lung-spleen (203), and lung-kidney (98). The known functions of the lung- prominent genes mainly fell into 5 categories: ligand binding, signal transducer, cell communication, development, and metabolism. Real-time PCR confirmed 13 lung-prominent genes, including 5 genes that have not been investigated in the lung, vitamin D-dependent calcium binding protein (Calb3), mitogen activated protein kinase 13 (Mapk13), solute carrier family 29 transporters, member 1 (Slc29a1), corticotropin releasing hormone receptor (Crhr1), and lipocalin 2 (Lcn2). Conclusion: The lung-prominent genes identified in this study may provide an important clue for further investigation of pulmonary functions. Background With the completion of genome projects of human and other model species, functional studies on a genomic scale are coming to a frontier. The investigation of tran- scriptome

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