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Identification of Endogenous Gibberellins from Sorghum1

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PMC
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  • Biology
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Abstract

Gibberellins (GA) A1, A19, and A20 were identified in shoot cylinders containing the apical meristems from sorghum (Sorghum bicolor L.). Extracts were purified by sequential SiO2 partition chromatography and reversed-phase C18 high performance liquid chromatography and biologically active (dwarf rice cv Tan-ginbozu microdrop assay) fractions were subjected to gas chromatography-selected ion monitoring. Based on the use of [3H]GA and [2H](d2)GA internal standards, amounts of GA1, GA19, and GA20 were estimated to be 0.7, 8.8, and 1.5 namograms per gram dry weight of tissue, respectively.

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