Coagulase-positive isolates of Staphylococcus intermedius from dogs and coagulase-positive isolates of Staphylococcus hyicus subsp. hyicus from a pig and cows were identified initially by a simplified scheme which can be readily performed in a routine diagnostic laboratory. Characters tested in the scheme included coagulase activity, colony pigment, and aerobic acid production from maltose. The identity of these isolates was confirmed by deoxyribonucleic acid-deoxyribonucleic acid hybridization experiments. The strains of S. hyicus subsp. hyicus isolated from porcine sources were positive for protein A, whereas the strains recovered from bovine mastitic milk were negative for protein A.