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Identification of a novel promoter from banana aquaporin family gene (MaTIP1;2) which responses to drought and salt-stress in transgenic Arabidopsis thaliana.

Authors
  • Song, Shun1
  • Xu, Yi2
  • Huang, Dongmei2
  • Miao, Hongxia3
  • Liu, Juhua3
  • Jia, Caihong3
  • Hu, Wei3
  • Valarezo, Ana Valeria2
  • Xu, Biyu4
  • Jin, Zhiqiang5
  • 1 Key Laboratory of Genetic Improvement of Bananas, Hainan Province, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China; National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research(Wuhan), Huazhong Agricultural University, Wuhan, China. , (China)
  • 2 Key Laboratory of Genetic Improvement of Bananas, Hainan Province, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. , (China)
  • 3 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. , (China)
  • 4 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. Electronic address: [email protected] , (China)
  • 5 Key Laboratory of Genetic Improvement of Bananas, Hainan Province, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Plant physiology and biochemistry : PPB
Publication Date
May 17, 2018
Volume
128
Pages
163–169
Identifiers
DOI: 10.1016/j.plaphy.2018.05.003
PMID: 29778840
Source
Medline
Keywords
License
Unknown

Abstract

Drought and salt stresses often affect plant growth and crop yields. Identification of promoters involved in drought and salt stress responses is of great significance for genetic improvement of crop resistance. Our previous studies showed that aquaporin can respond to drought and salt stresses, but its promoter has not yet been reported in plants. In the present study, cis-acting elements of MaAQP family member promoters were systematically analyzed in banana. Expression of MaTIP1; 2 was induced by drought and salt stresses but not sensitive to cold stress, waterlogging stress, or mechanical damage, and its promoter contained five stress-related cis-acting elements. The MaTIP1; 2 promoter (841 bp upstream of translation initiation site) from banana (Musa acuminata L. AAA group cv. Brazilian) was isolated through genome walking polymerase chain reaction, and found to contain a TATA Box, CAAT box, ABRE element, CCGTCC box, CGTCA motif, and TCA element. Transformation of the MaTIP1; 2 promoter into Arabidopsis to assess its function indicated that it responds to both drought and salt stress treatments. These results suggest that MaTIP1; 2 utilization may improve drought and salt stresses resistance of the transgenic plants by promoting banana aquaporin expression.

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