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Identification of the N-acetylcysteine conjugate of benzidine formed in the peroxidase activation system.

Authors
  • Josephy, P D
  • Iwaniw, D C
Type
Published Article
Journal
Carcinogenesis
Publication Date
Jan 01, 1985
Volume
6
Issue
1
Pages
155–158
Identifiers
PMID: 3967336
Source
Medline
License
Unknown

Abstract

Benzidine is oxidized by the peroxidase/H2O2 system, yielding reactive intermediates. In the presence of thiols, covalent adducts are formed. We used h.p.l.c. to separate the products of the reaction of benzidine with N-acetylcysteine. The major product was identified by n.m.r. spectroscopy (1H-n.m.r.) as 3-(N-acetylcystein-S-yl)-benzidine.

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