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Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli.

Authors
  • Graupner, S
  • Wackernagel, W
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
May 01, 1996
Volume
62
Issue
5
Pages
1839–1841
Identifiers
PMID: 8633885
Source
Medline
License
Unknown

Abstract

Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.

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