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Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor.

Authors
  • Do, Hyun-Jin
  • Song, Hyuk
  • Yang, Heung-Mo
  • Kim, Dong-Ku
  • Kim, Nam-Hyung
  • Kim, Jin-Hoi
  • Cha, Kwang-Yul
  • Chung, Hyung-Min
  • Kim, Jae-Hwan
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Mar 20, 2006
Volume
580
Issue
7
Pages
1865–1871
Identifiers
PMID: 16516205
Source
Medline
License
Unknown

Abstract

We investigated nuclear localization signal (NLS) determinants within the AT-hook and ETS DNA-binding domains of murine Elf3 (mElf3), a member of the subfamily of epithelium-specific ETS transcription factors. Deletion mutants containing the AT-hook, ETS domain or both localized strictly in the nucleus, suggesting that these individual domains contain independent NLS motif(s). Within the AT-hook domain, four basic residues (244KRKR247) were critical for strong NLS activity, and two potent bipartite NLS motifs (236-252 and 249-267) were sufficient for nuclear import of mElf3, although less efficient than the full domain. In addition, one stretch of basic residues (318KKK320) within the ETS domain appears to be essential for mElf3 nuclear localization. Taken together, mElf3 contains multiple NLS motifs, which may function cooperatively to effect efficient nuclear transport.

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