Affordable Access

Identification of a long form of cystatin from human saliva by rapid microbore HPLC mapping.

Authors
  • Hawke, D H
  • Yuan, P M
  • Wilson, K J
  • Hunkapiller, M W
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Jun 30, 1987
Volume
145
Issue
3
Pages
1248–1253
Identifiers
PMID: 3496880
Source
Medline
License
Unknown

Abstract

Microbore HPLC methodology permits rapid and sensitive mapping of human saliva proteins. Saliva is sampled and processed in less than one hour, greatly reducing the likelihood of artifactual protein degradation. As little as 50 microliters of saliva yields proteins in sufficient quantities and purity to obtain amino terminal sequences directly. By this route we have discovered a 14 kDa protein extremely homologous to Cystatin S, but amino-terminally extended by eight amino acids.

Report this publication

Statistics

Seen <100 times