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Identification of a linear B-cell epitope on non-structural protein 12 of porcine reproductive and respiratory syndrome virus, using a monoclonal antibody

Authors
  • Bi, Caihong1
  • Shao, Zengyu1
  • Zhang, Yuanfeng2
  • Hu, Liang1
  • Li, Jiangnan1
  • Huang, Li1
  • Weng, Changjiang1
  • 1 Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Biotechnology, Harbin, 150001, China , Harbin (China)
  • 2 Yangtze University, College of Life Sciences, Jingzhou, 434100, China , Jingzhou (China)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Apr 01, 2017
Volume
162
Issue
8
Pages
2239–2246
Identifiers
DOI: 10.1007/s00705-017-3355-8
Source
Springer Nature
Keywords
License
Yellow

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) has caused tremendous economic losses and continues to be a serious problem to the swine industry worldwide. The structure and function of PRRSV nonstructural protein 12 (NSP12) is still unknown. In this study, we produced a monoclonal antibody, named as 1E5, against the NSP12 protein of HP (highly pathogenic) -PRRSV strain HuN4. A series of partially overlapping recombinant NSP12 truncations and synthesized peptides were used to define the epitope recognized by 1E5. We found that 130KANATSMRFH139 is the minimal linear epitope and that it is highly conserved among some HP-PRRSV isolates of type 2 PRRSV, but not the classical isolates of type 2 PRRSV or the isolates of type 1 PRRSV. Therefore, 1E5 can be used to establish a valuable tool to distinguish infections with HP-PRRSV isolates of type 2 PRRSV from the classical isolates of type 2 PRRSV and type 1 PRRSV.

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