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Identification and the immunological role of two Nimrod family genes in the silkworm, Bombyx mori.

Authors
  • Gul, Isma1
  • Kausar, Saima1
  • You, Qiuxiang2
  • Sun, Wei1
  • Li, Zekun1
  • Abbas, Muhammad Nadeem3
  • Cui, Hongjuan4
  • 1 State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China. , (China)
  • 2 Neurological Disease Center of the Third Affiliated Hospital of Chongqing Medical University, 401120, China. , (China)
  • 3 State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China. Electronic address: [email protected] , (China)
  • 4 State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Oct 21, 2021
Volume
193
Issue
Pt A
Pages
154–165
Identifiers
DOI: 10.1016/j.ijbiomac.2021.10.083
PMID: 34688681
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In animals, immune signaling pathways and effector molecules participate in attenuating microbial infection. Recent work has shown that the Nimrod family proteins can directly bind to bacteria, and this binding leads to bacterial phagocytosis. Although the Nimrod gene family has been reported in many non-drosophilids, their functions remain unexplored in most insect species. Here, we report two members (Nimrod-B and Draper) of the Nimrod gene family from Bombyx mori and analyzed their role in immunity. The two genes were ubiquitously expressed in the tested tissues; but, they transcribed preferentially in immune tissues. The developmental profiles showed that BmNimrod-B and BmDraper transcription levels were highest in the pupal stages. Challenge with microbial pathogens induced the transcription levels of all two genes at different time points. Knockdown of BmDraper decreased the bacterial clearance and increased their replication relative to the control group, whereas, BmNimrod-B suppression had a non-significant effect on them. Furthermore, the mortality rate was increased after BmDraper silencing. The knockdown of these genes did not significantly affect the production of antimicrobial peptides following E. coli infection. Taken together, the Nimrod family genes play a crucial role in host defense by positively regulating the antibacterial immune response in silkworm B. mori. Copyright © 2021 Elsevier B.V. All rights reserved.

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