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Identification of hydrogen selenide and other volatile selenols by derivatization with 1-fluoro-2,4-dinitrobenzene

Authors
  • Ganther, H.E.
  • Kraus, R.J.
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1984
Volume
138
Issue
2
Pages
396–403
Identifiers
DOI: 10.1016/0003-2697(84)90828-5
Source
Elsevier
Keywords
License
Unknown

Abstract

A procedure is described for the trapping and identification of hydrogen selenide and methyl selenol (CH 3SeH). The volatile selenols were generated by reducing selenious acid or dimethyldiselenide with Zn dust and hydrochloric acid under a stream of nitrogen and passing into a trapping solution compoosed of 50 m m 1-fluoro-2,4-dinitrobenzene plus 83 m m sodium bicarbonate in 67% dimethylformamide:33% water. The selenols react rapidly to form stable dinitrophenyl (DNP) selenoethers that can be extracted into benzene; these are easily identified by TLC, HPLC, or mass spectrometry. Hydrogen selenide is trapped in 90–99% yield, primarily as the di-DNP-monoselenide with a trace of di-DNP-diselenide.

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