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Identification of human rotavirus strains with the P[14] genotype by PCR.

Authors
  • Arista, S
  • Vizzi, E
  • Alaimo, C
  • Palermo, D
  • Cascio, A
Type
Published Article
Journal
Journal of clinical microbiology
Publication Date
Aug 01, 1999
Volume
37
Issue
8
Pages
2706–2708
Identifiers
PMID: 10405427
Source
Medline
License
Unknown

Abstract

A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].

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