Affordable Access

Access to the full text

Identification and Functional Analysis of the G1 Phase Cyclin Dependent Kinase Gene Hc-CDK6 in Pearl Mussels (Hyriopsis cumingii)

Authors
  • Feng, ShangLe1
  • Li, XueNan1
  • Wang, He1
  • Li, WenJuan1, 2, 3
  • Bai, ZhiYi1, 2, 3
  • 1 Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai , (China)
  • 2 Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai , (China)
  • 3 Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals, Shanghai , (China)
Type
Published Article
Journal
Frontiers in Marine Science
Publisher
Frontiers Media S.A.
Publication Date
Jun 01, 2022
Volume
9
Identifiers
DOI: 10.3389/fmars.2022.921726
Source
Frontiers
Keywords
Disciplines
  • Marine Science
  • Original Research
License
Green

Abstract

Cyclin dependent kinase 6 (CDK6) is a serine/threonine kinase that plays important roles in cell cycle progression and differentiation. In this study, full-length cDNA of Hc-CDK6 was obtained from freshwater pearl mussels (Hyriopsis cumingii, Hc) with 3´,5´ rapid-amplification of cDNA ends (RACE). The Hc-CDK6 expression profiles were analyzed with quantitative real-time PCR and in situ hybridization. The function of the Hc-CDK6 gene was studied with both RNA interference (RNAi) and overexpression in H. cumingii. Hc-CDK6 was found to encode 331 amino acids and to have a CDK4/6-like serine/threonine kinase catalytic structural domain. In terms of the amino acid sequence, the protein Hc-CDK6 was most closely related to its homolog in Crassostrea gigas, with a similarity of 75.23%. Hc-CDK6 was expressed in all examined tissues (adductor, foot, visceral mass, gill, outer mantle, inner mantle and gonads), and the highest expression was observed in the gonads (P<0.05). The relative expression of Hc-CDK6 increased during embryonic development, and was higher in the blastocyst and gastrulation stages, which were characterized by rapid division and differentiation. Hc-CDK6 showed hybridization signals in all parts of the mantle. After knockdown of Hc-CDK6 through RNAi, a significant decrease in CDK6 expression was found, and the percentage of cells in G0/G1 significantly increased. Overexpression of Hc-CDK6 in mantle cells increased the proliferation of cultured cells (P<0.05). Hc-CDK6 appeared to promote the cell cycle in H. cumingii, and overexpression of Hc-CDK6 promoted mantle cell proliferation. The functional study of this gene may provide new ideas for solving the problem of slow proliferation of shellfish cells in in vitro culture.

Report this publication

Statistics

Seen <100 times