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Identification, expression and functional characterisation of CYP1A in grass carp (Ctenopharyngodon idella).

Authors
  • Chu, Pengfei1
  • He, Libo2
  • Zhu, Denghui1
  • Huang, Rong2
  • Liao, Lanjie2
  • Li, Yongming2
  • Zhu, Zuoyan2
  • Wang, Yaping3
  • 1 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China. , (China)
  • 2 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. , (China)
  • 3 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Fish & Shellfish Immunology
Publisher
Elsevier
Publication Date
Oct 11, 2019
Volume
95
Pages
35–43
Identifiers
DOI: 10.1016/j.fsi.2019.10.022
PMID: 31610292
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In mammal, CYP1A has attracted special attention due to its important roles in the oxidative metabolism. In fish, the researches on CYP1A are more focus on its roles in pollution in water environments, but the immune function is unclear. In the study, CiCYP1A gene was cloned from grass carp (Ctenopharyngodon idella). Tissue distribution exhibited an overwhelmingly high basal expression levels in the liver. After GCRV infection, CiCYP1A showed a potent response, indicating CiCYP1A was involved in GCRV-induced immunity. Subcellular localisation showed CiCYP1A was distributed in the cytoplasm. Besides, dual-luciferase activity assays revealed CYP1A was relevant for IFN-I signaling pathway modulation, furthermore, overexpressed CYP1A potently suppressed the mRNA expression of IRF3 and IFN-I but not IRF7. The results provide new sights into exploring immune function of CiCYP1A in teleosts. Copyright © 2019. Published by Elsevier Ltd.

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