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Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus

Authors
  • Liu, Wenming1
  • Yang, Baolin1
  • Wang, Mingxia1
  • Liang, Weifeng1
  • Wang, Haiwei1
  • Yang, Decheng1
  • Ma, Wenge2
  • Zhou, Guohui1
  • Yu, Li1
  • 1 Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People’s Republic of China , Harbin (China)
  • 2 Xinjiang Academy of Animal Science, Institute of Veterinary Medicine, 151 Eastern Kelamayi Street, Urumqi, 830000, People’s Republic of China , Urumqi (China)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Mar 03, 2017
Volume
162
Issue
7
Pages
1877–1885
Identifiers
DOI: 10.1007/s00705-017-3304-6
Source
Springer Nature
Keywords
License
Yellow

Abstract

Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, a potent FMDV serotype-independent monoclonal antibody (MAb) 3D9 was generated. Screening of a phage-displayed random 12-peptide library revealed that MAb 3D9 bound to phages displaying a consensus motif GVYxxAYxW that is highly homologous to the 89GVYxxxxxxxAYxxxxW105 motif in the FMDV VP2 protein. Importantly, this conformational epitope was highly conserved among all seven serotypes of FMDV analyzed in sequence alignments. To further verify the authentic epitope recognized by MAb 3D9, a FMDV O/YS/CHA/05 mutant virus V90A was generated using a reverse genetics system. The results revealed that Val90 was an important residue for MAb 3D9 binding within this conformational epitope. Thus, we finely mapped a conserved conformational epitope onto the FMDV VP2 protein. These results could be applied in the development of epitope-based vaccines and suitable MAb-based diagnostic methods for various FMDV serotype-independent tests.

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