Affordable Access

deepdyve-link
Publisher Website

Identification of the citrate exporter Cex1 of Yarrowia lipolytica.

Authors
  • Erian, Anna Maria1, 2
  • Egermeier, Michael1, 2
  • Rassinger, Alice2, 3
  • Marx, Hans1, 2
  • Sauer, Michael1, 2, 3
  • 1 CD-Laboratory for Biotechnology of Glycerol, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria. , (Austria)
  • 2 Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria. , (Austria)
  • 3 Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, 1190 Vienna, Austria. , (Austria)
Type
Published Article
Journal
FEMS Yeast Research
Publisher
Oxford University Press
Publication Date
Oct 15, 2020
Volume
20
Issue
7
Identifiers
DOI: 10.1093/femsyr/foaa055
PMID: 32990722
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Yarrowia lipolytica is a yeast with many talents, one of them being the production of citric acid. Although the citrate biosynthesis is well studied, little is known about the transport mechanism by which citrate is exported. To gain better insight into this mechanism, we set out to identify a transporter involved in citrate export of Y. lipolytica. A total of five proteins were selected for analysis based on their similarity to a known citrate exporter, but neither a citrate transport activity nor any other phenotypic function could be attributed to them. Differential gene expression analysis of two strains with a distinct citrate productivity revealed another three putative transporters, one of which is YALI0D20196p. Disrupting YALI0D20196g in Y. lipolytica abolished citrate production, while extrachromosomal expression enhanced citrate production 5.2-fold in a low producing wildtype. Furthermore, heterologous expression of YALI0D20196p in the non-citrate secreting yeast Saccharomyces cerevisiae facilitated citrate export. Likewise, expression of YALI0D20196p complemented the ability to secrete citrate in an export-deficient strain of Aspergillus niger, confirming a citrate export function of YALI0D20196p. This report on the identification of the first citrate exporter in Y. lipolytica, termed Cex1, represents a valuable starting point for further investigations of the complex transport processes in yeasts. © The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.

Report this publication

Statistics

Seen <100 times