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Identification and characterization of three microsporidian genera concurrently infecting a silkworm, Bombyx mori, in Brazil.

Authors
  • Cassal, Maximiano Corrêa1
  • Fukushima, Airi2
  • Nishi, Oumi3
  • Iiyama, Kazuhiro4
  • Fiuza, Lidia Mariana5
  • Yasunaga-Aoki, Chisa6
  • 1 Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan. Electronic address: [email protected] , (Japan)
  • 2 Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan. Electronic address: [email protected] , (Japan)
  • 3 Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan. Electronic address: [email protected] , (Japan)
  • 4 Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan. Electronic address: [email protected] , (Japan)
  • 5 CABIO - Control Agro Bio Agricultural Research and Defence Ltd., 90670-100 Porto Alegre, RS, Brazil. Electronic address: [email protected] , (Brazil)
  • 6 Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, 819-0395 Fukuoka, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Journal of Invertebrate Pathology
Publisher
Elsevier
Publication Date
Nov 01, 2020
Volume
177
Pages
107502–107502
Identifiers
DOI: 10.1016/j.jip.2020.107502
PMID: 33197450
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Microsporidia are important entomopathogens known for infecting insects such as the silkworm (Bombyx mori) thus impairing global silk production. This study aimed to identify and characterize the microsporidia isolated from a diseased larva of silkworm, collected from a sericulture farm in southern Brazil. Identification was performed by phylogenetic analysis of the nucleotide sequences of the SSU rRNA genes. Characterization was performed by analyzing spore sizes, tissue tropism, internal and external symptoms, and pathogenicity against B. mori. Microsporidia belonging to three different genera were identified, namely, Endoreticulatus, Nosema and Tubulinosema. After inoculation of the mixed spores of the microsporidian isolates into B. mori larvae, a high prevalence of Tubulinosema spp. was observed. This isolate showed high prevalence on the silk glands and a late mortality, initially of around 10% until the 20th day post-inoculation but reaching 91.5% upon pupation. Therefore, we demonstrated that Tubulinosema spp. causes chronic infection with slow pathogenicity. We identified for the first time three different microsporidians concurrently infecting B. mori in Brazil. Tubulinosema is of particular interest because of its potential threat to silk production; it affects the formation of silk glands in B. mori while not presenting distinguishable external symptoms or causing the immediate death of these insects. Further studies focusing on this species, mainly regarding its life cycle within the host and the sublethal effects of surviving individuals, demonstrate the importance of describing it as a new species and improving the characterization of the disease in order to prevent its spread. Copyright © 2020 Elsevier Inc. All rights reserved.

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