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Identification and characterization of permissive cells to dengue virus infection in human hematopoietic stem and progenitor cells.

Authors
  • Hsu, Alan Y1, 2, 3
  • Ho, Tzu-Chuan1, 2
  • Lai, Mei-Ling1
  • Tan, Sia Seng1
  • Chen, Tsai-Yun4
  • Lee, Meed1
  • Chien, Yu-Wen5
  • Chen, Ya-Ping4
  • Perng, Guey Chuen1, 2
  • 1 Department of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan. , (Taiwan)
  • 2 Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan. , (Taiwan)
  • 3 Department of Biological Sciences, Purdue University, West Lafayette, Indiana. , (India)
  • 4 Division of Hematology and Oncology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. , (Taiwan)
  • 5 Departement of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan. , (Taiwan)
Type
Published Article
Journal
Transfusion
Publication Date
Sep 01, 2019
Volume
59
Issue
9
Pages
2938–2951
Identifiers
DOI: 10.1111/trf.15416
PMID: 31251408
Source
Medline
Language
English
License
Unknown

Abstract

Dengue virus (DENV) is a significant threat to public health in tropical and subtropical regions, where the frequency of human migration is increasing. Transmission of DENV from donors to recipients after hematopoietic stem cell transplantation has been steadily described. However, the underlying mechanisms remain unclear. Freshly isolated bone marrow (BM) was subjected to DENV infection, followed by multicolor fluorescence-activated cell sorting (FACS) analysis. Virus in supernatants was collected and analyzed by plaque assay. DENV-1 to DENV-4 could effectively infect freshly obtained BM and produced infectious virus. DENV infection did not change the quantitative population of hematopoietic stem and progenitor cells (HSPCs), megakaryocytic progenitor cells (MkPs) and megakaryocytes. Additionally, DENV antigen, nonstructural protein 1, was enriched in HSPCs and MkPs of DENV infected marrow cells. CD34+, CD133+, or CD61+ cells sorted out from BM were not only the major contributing targets facilitating the DENV infection directly but also facilitated the spread of DENV into other cells when cocultured. Results suggest that DENV can efficiently infect HSPCs, which might jeopardize the recipients if DENV-infected cells were subsequently used. We therefore raise the need for DENV screening for both the donors and recipients of hematopoietic stem cell transplantation, especially for donors exposed to endemic areas, to mitigate DENV infection in immunocompromised recipients. © 2019 AABB.

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