Affordable Access

deepdyve-link
Publisher Website

Identification and characterization of a novel Apical Membrane Antigen 3 in Eimeria tenella.

Authors
  • Wang, Qingjie1
  • Zhu, Shunhai1
  • Zhao, Qiping1
  • Huang, Bing1
  • Yu, Shuilan1
  • Yu, Yu1
  • Liang, Shanshan1
  • Wang, Haixia1
  • Zhao, Huanzhi1
  • Han, Hongyu1
  • Dong, Hui1
  • 1 Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute CAAS, Shanghai, 200241, China. , (China)
Type
Published Article
Journal
The Journal of eukaryotic microbiology
Publication Date
Dec 05, 2020
Identifiers
DOI: 10.1111/jeu.12836
PMID: 33289220
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Eimeria tenella is an obligate intracellular parasite in the phylum Apicomplexa. As described for other members of Apicomplexa, apical membrane antigen 1 (AMA1) has been shown to be critical for sporozoite invasion of host cells by E. tenella. Recently, an E. tenella paralogue of AMA1 (EtAMA1), dubbed sporoAMA1 (EtAMA3), was identified in proteomic and transcriptomic analyses of E. tenella, but not further characterized. Here, we show that EtAMA3 is a type I integral membrane protein that has 24% -38% identity with other EtAMAs. EtAMA3 has the same pattern of Cys residues in domains I and II of AMA1 orthologs from apicomplexan parasites, but high variance in domain III, with all six invariant Cys residues absent. EtAMA3 expression was developmentally regulated at the mRNA and protein levels. EtAMA3 protein was detected in sporulated oocysts and sporozoites, but not in the unsporulated oocysts or second generation merozoites. EtAMA3 is secreted by micronemes and is primarily localized to the apical end of sporozoites during host-cell invasion. Additionally, pretreatment of sporozoites with rEtAMA3-specific antibodies substantially impeded their invasion into host cells. These results suggest EtAMA3 is a sporozoite-specific protein that is involved in host-cell sporozoite invasion. This article is protected by copyright. All rights reserved.

Report this publication

Statistics

Seen <100 times