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Identification and characterization of low molecular weight polyphenols in berry leaf extracts by HPLC-DAD and LC-ESI/MS.

Authors
  • Oszmiański, Jan1
  • Wojdyło, Aneta
  • Gorzelany, Józef
  • Kapusta, Ireneusz
  • 1 Department of Fruit, Vegetable and Grain Technology, Wroclaw University of Environmental and Life Sciences, 37/41 Chełmońskiego Street, 51-630 Wroclaw, Poland. [email protected] , (Poland)
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Dec 28, 2011
Volume
59
Issue
24
Pages
12830–12835
Identifiers
DOI: 10.1021/jf203052j
PMID: 22098480
Source
Medline
License
Unknown

Abstract

This paper reports the results of qualitative and quantitative analyses of low molecular weight phenolics from five berry leaf extract using HPLC-DAD and LC-ESI/MS. The identification of the black currant, raspberry, bilberry, honeysuckle, and strawberry leaf phytochemicals was based on the comparison of UV-vis absorption maxima (λ(max)) and mass spectral analysis. The peak identification in samples was also based on comparisons of the retention times (t(R)) of the isolated phytochemical standards. Knowledge of the precise phenolic profile of berry leaves may offer a scientific basis to put the underutilized berry leaves to good use as very cheap raw materials for polyphenol extract production. These studies indicated that these leaves can be used as a good and cheap source of bioactive constituents. These results suggest that berry leaves are a potential source of phenolics and have potential pro-healthy properties to contribute to human health.

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