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Identification and characterization of an interleukin-15 homologue from Tetraodon nigroviridis.

Authors
  • Fang, Wei
  • Xiang, Li-Xin
  • Shao, Jian-Zhong
  • Wen, Yi
  • Chen, Shu-Yun
Type
Published Article
Journal
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Publisher
Elsevier
Publication Date
Mar 01, 2006
Volume
143
Issue
3
Pages
335–343
Identifiers
PMID: 16455279
Source
Medline
License
Unknown

Abstract

Interleukin-15 (IL-15) plays an important role in adaptive immune systems in vertebrates with similar bioactivities to interleukin-2 (IL-2). Here we report molecular cloning, sequence analysis and distribution of an IL-15 homologue from a pufferfish (Tetraodon nigroviridis). It is located within a 3,088 bp genomic fragment, transcribed into a 1,056 bp mRNA including 158 bp 5'UTR (untranslated region), 519 bp ORF (open reading frame) and 379 bp 3'UTR. T. nigroviridis IL-15 is constitutively detectable in tissues and organs selected. Levels of transcripts were observed after various stimulations. Gene organization is similar to mammals and birds, and a high degree of conservation of chromosome synteny exists between them. Systematic genomics search against Takifugu rubripes genome supports our conclusions. The T. nigroviridis IL-15 precursor with 172aa (amino acids) contains a putative 53aa signal peptide, while the mature peptide has a calculated molecular mass of 13.36 kDa and a theoretical pI of 4.67. The protein sequence shares 13.3-62.1% identity with reported IL-15s. Phylogenetic analysis grouped Tetraodon with other fish on a separated branch, excluded from mammalian and avian IL-15s. In addition, our analysis on another annotated T. nigroviridis IL-15 demonstrated that it may be a paralogue of IL-15. To differentiate it from the known IL-15s, we described it as IL-15x.

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