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Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato

Authors
  • Sierra-Orozco, Edgar1
  • Shekasteband, Reza2
  • Illa-Berenguer, Eudald3
  • Snouffer, Ashley3
  • van der Knaap, Esther3, 4
  • Lee, Tong Geon1
  • Hutton, Samuel F.1
  • 1 University of Florida, Gulf Coast Research and Education Center, 14625 County Road 672, Wimauma, FL, 33598, USA , Wimauma (United States)
  • 2 North Carolina State University, Department of Horticultural Science, Mountain Horticultural Crops Research and Extension Center, 455 Research Drive, Mills River, NC, 28759, USA , Mills River (United States)
  • 3 Center for Applied Genetic Technologies, 111 Riverbend Road, Athens, GA, 30602, USA , Athens (United States)
  • 4 Department of Horticulture, 1111 Plant Sciences Bldg, Athens, GA, 30602, USA , Athens (United States)
Type
Published Article
Journal
Horticulture Research
Publisher
Nature Publishing Group UK
Publication Date
Jun 01, 2021
Volume
8
Issue
1
Identifiers
DOI: 10.1038/s41438-021-00574-3
Source
Springer Nature
License
Green

Abstract

Within large-fruited germplasm, fruit size is influenced by flat and globe shapes. Whereas flat fruits are smaller and retain better marketability, globe fruits are larger and more prone to cuticle disorders. Commercial hybrids are often developed from crosses between flat and globe shaped parents because flat shape is thought to be dominant and fruit size intermediate. The objectives of this study were to determine the genetic basis of flat/globe fruit shape in large-fruited fresh-market tomato germplasm and to characterize its effects on several fruit traits. Twenty-three advanced single plant selections from the Fla. 8000 × Fla. 8111B cross were selectively genotyped using a genome-wide SNP array, and inclusive composite interval mapping identified a single locus on the upper arm of chromosome 12 associated with shape, which we termed globe. A 238-plant F2 population and 69 recombinant inbred lines for this region from the same parents delimited globe to approximately 392-kilobases. A germplasm survey representing materials from multiple breeding programs demonstrated that the locus explains the flat/globe shape broadly. A single base insertion in an exon of Solyc12g006860, a gene annotated as a brassinosteroid hydroxylase, segregated completely with shape in all populations tested. CRISPR/Cas9 knock out plants confirmed this gene as underlying the globe locus. In silico analysis of the mutant allele of GLOBE among 595 wild and domesticated accessions suggested that the allele arose very late in the domestication process. Fruit measurements in three genetic backgrounds evidenced that globe impacts fruit size and several fruit shape attributes, pedicel length/width, and susceptibility of fruit to weather check. The mutant allele of GLOBE appears mostly recessive for all traits except fruit size where it acts additively.

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