Affordable Access

Access to the full text

Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower

Authors
  • Zhao, Zhenqing
  • Sheng, Xiaoguang
  • Yu, Huifang
  • Wang, Jiansheng
  • Shen, Yusen
  • Gu, Honghui
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Mar 15, 2020
Volume
21
Issue
6
Identifiers
DOI: 10.3390/ijms21061999
PMID: 32183438
PMCID: PMC7139996
Source
PubMed Central
Keywords
License
Green

Abstract

“Riceyness” refers to the precocious development of flower bud initials over the curd surface of cauliflower, and it is regarded as undesirable for the market. The present study aimed to identify the candidate loci and genes responsible for the morphological difference in riceyness between a pair of cauliflower sister lines. Genetic analysis revealed that riceyness is controlled by a single dominant locus. An F2 population derived from the cross between these sister lines was used to construct “riceyness” and “non-riceyness” bulks, and then it was subjected to BSA-seq. On the basis of the results of Δ(SNP-index) analysis, a 4.0 Mb candidate region including 22 putative SNPs was mapped on chromosome C04. Combining the RNA-seq, gene function annotation, and target sequence analysis among two parents and other breeding lines, an orthologous gene of the Arabidopsis gene SOC1 , Bo4g024850 was presumed as the candidate gene, and an upstream SNP likely resulted in riceyness phenotype via influencing the expression levels of Bo4g024850 . These results are helpful to understand the genetic mechanism regulating riceyness, and to facilitate the molecular improvement on cauliflower curds.

Report this publication

Statistics

Seen <100 times