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Identification of Bacillus megaterium and Microbacterium liquefaciens genes involved in metal resistance and metal removal.

Authors
  • Fierros-Romero, Grisel1, 1
  • Gómez-Ramírez, Marlenne1, 1
  • Arenas-Isaac, Ginesa E1, 1
  • Pless, Reynaldo C1, 1
  • Rojas-Avelizapa, Norma G1, 1
  • 1 Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Cerro Blanco 141, Col. Colinas del Cimatario, Querétaro, Querétaro 76090, Mexico. , (Mexico)
Type
Published Article
Journal
Canadian Journal of Microbiology
Publisher
Canadian Science Publishing
Publication Date
Jun 01, 2016
Volume
62
Issue
6
Pages
505–513
Identifiers
DOI: 10.1139/cjm-2015-0507
PMID: 27210016
Source
Medline
Keywords
License
Unknown

Abstract

Bacillus megaterium MNSH1-9K-1 and Microbacterium liquefaciens MNSH2-PHGII-2, 2 nickel- and vanadium-resistant bacteria from mine tailings located in Guanajuato, Mexico, are shown to have the ability to remove 33.1% and 17.8% of Ni, respectively, and 50.8% and 14.0% of V, respectively, from spent petrochemical catalysts containing 428 ± 30 mg·kg(-1) Ni and 2165 ± 77 mg·kg(-1) V. In these strains, several Ni resistance determinants were detected by conventional PCR. The nccA (nickel-cobalt-cadmium resistance) was found for the first time in B. megaterium. In M. liquefaciens, the above gene as well as the czcD gene (cobalt-zinc-cadmium resistance) and a high-affinity nickel transporter were detected for the first time. This study characterizes the resistance of M. liquefaciens and B. megaterium to Ni through the expression of genes conferring metal resistance.

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