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Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes

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American Society for Microbiology
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PMC
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Abstract

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

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