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Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes

Authors
  • F. M. Thibault
  • E. Valade
  • D. R. Vidal
Publisher
American Society for Microbiology
Publication Date
Dec 01, 2004
Source
PMC
Keywords
License
Unknown

Abstract

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

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