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Identification and Analysis of Different Types of UFBs.

Authors
  • Gemble, Simon1
  • Amor-Guéret, Mounira2, 3
  • 1 Institut Curie, PSL Research University, CNRS UMR144, Paris, France. , (France)
  • 2 Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, France. [email protected] , (France)
  • 3 CNRS UMR 3348, Paris Saclay University, Institut Curie, Research Center, Orsay, France. [email protected] , (France)
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2021
Volume
2153
Pages
187–192
Identifiers
DOI: 10.1007/978-1-0716-0644-5_13
PMID: 32840780
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Ultrafine anaphase bridges (UFBs) result from a defect in sister chromatid segregation during anaphase. They arise from particular DNA structures, mostly generated at specific loci in the human genome, such as centromeres, common fragile sites, telomeres, or ribosomal DNA. Increases in UFB frequency are a marker of genetic instability, and their detection has become a classic way of detecting such genetic instability over the last decade. Here we describe a protocol to stain different types of UFBs in adherent human cells.

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