Affordable Access

deepdyve-link
Publisher Website

Hypoxia modulates human mast cell adhesion to hyaluronic acid.

Authors
  • Pastwińska, Joanna1, 2
  • Walczak-Drzewiecka, Aurelia1
  • Kozłowska, Elżbieta2
  • Harunari, Enjuro3
  • Ratajewski, Marcin4
  • Dastych, Jarosław5
  • 1 Laboratory of Cellular Immunology, Institute of Medical Biology, Polish Academy of Sciences, 90-364, Lodz, Poland. , (Poland)
  • 2 Department of Experimental Immunology, Medical University of Lodz, 92-213, Lodz, Poland. , (Poland)
  • 3 Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan. , (Japan)
  • 4 Laboratory of Epigenetics, Institute of Medical Biology, Polish Academy of Sciences, 90-364, Lodz, Poland. , (Poland)
  • 5 Laboratory of Cellular Immunology, Institute of Medical Biology, Polish Academy of Sciences, 90-364, Lodz, Poland. [email protected] , (Poland)
Type
Published Article
Journal
Immunologic research
Publication Date
Apr 01, 2022
Volume
70
Issue
2
Pages
152–160
Identifiers
DOI: 10.1007/s12026-021-09228-x
PMID: 34791576
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Hypoxia is an inherent factor in the inflammatory process and is important in the regulation of some immune cell functions, including the expression of mast cell pro- and anti-inflammatory mediators. Hypoxia also influences cell adhesion to the extracellular matrix (ECM). Hyaluronic acid is one of the major components of the ECM that is involved in inflammatory and tissue regeneration processes in which mast cells play a prominent role. This prompted us to investigate the effects of hypoxia on the expression of hyaluronic acid receptors in mast cells and mast cell adhesion to this ECM component. We found that human LAD2 mast cells spontaneously adhered to hyaluronic acid in a CD44-dependent manner and that reduced oxygen concentrations inhibited or even completely abolished this adhesion process. The mechanism of hypoxia downregulation of mast cell adhesion to hyaluronic acid did not involve a decrease in CD44 expression and hyaluronidase-mediated degradation of adhesion substrates but rather conformational changes in the avidity of CD44 to hyaluronic acid. Hypoxia-mediated regulation of mast cell adhesion to extracellular matrix components might be involved in the pathogenic accumulation of mast cells observed in the course of certain diseases including rheumatoid arthritis and cancer. © 2021. The Author(s).

Report this publication

Statistics

Seen <100 times