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Hyphenation of ultra high performance supercritical fluid chromatography with atmospheric pressure chemical ionisation high resolution mass spectrometry: Part 1. Study of the coupling parameters for the analysis of natural non-polar compounds.

Authors
  • Duval, Johanna1
  • Colas, Cyril2
  • Pecher, Virginie3
  • Poujol, Marion3
  • Tranchant, Jean-François3
  • Lesellier, Eric4
  • 1 Univ. Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France. , (France)
  • 2 Univ. Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France; CNRS, CBM, UPR 4301, Univ. Orléans, F-45071 Orléans, France. , (France)
  • 3 LVMH Recherche, 185 avenue de Verdun, F-45800 Saint-Jean-de-Braye, France. , (France)
  • 4 Univ. Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Aug 04, 2017
Volume
1509
Pages
132–140
Identifiers
DOI: 10.1016/j.chroma.2017.06.016
PMID: 28619592
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

An analytical method based on Ultra-High-Performance Supercritical Fluid Chromatography (UHPSFC) coupled with Atmospheric Pressure Chemical Ionization - High-resolution mass spectrometry (APCI-Q-TOF-HRMS) was developed for compounds screening from oily samples. The hyphenation was made using a commercial UHPLC device coupled to a CO2 pump in order to perform the chromatographic analysis. An adaptation of the injection system for compressible fluids was accomplished for this coupling: this modification of the injection sequence was achieved to prevent unusual variations of the injected volume related to the use of a compressible fluid. UHPSFC-HRMS hyphenation was optimized to enhance the response of the varied compounds from a seed extract (anthraquinones, free fatty acids, diacylglycerols, hydroxylated triacylglycerols and triacylglycerols). No split was used prior to the APCI ionization source, allowing introducing all the compounds in the spectrometer, ensuring a better sensitivity for minor compounds. The effects of a mechanical make-up (T-piece) added before this ionization source was discussed in terms of standard deviation of response, response intensity and fragmentation percentage. The location of the T-piece with regards to the backpressure regulator (BPR), the flow rate and the nature of the make-up solvent were studied. Results show that the effects of the studied parameters depend on the nature of the compounds, whereas the make-up addition favours the robustness of the mass response (quantitative aspect). Copyright © 2017 Elsevier B.V. All rights reserved.

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