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Hyperglycosylation of glycopeptidolipid of Mycobacterium smegmatis under nutrient starvation: structural studies.

Authors
  • Mukherjee, Raju
  • Gomez, Manuel
  • Jayaraman, Narayanaswamy
  • Smith, Issar
  • Chatterji, Dipankar
Type
Published Article
Journal
Microbiology (Reading, England)
Publication Date
Jul 01, 2005
Volume
151
Issue
Pt 7
Pages
2385–2392
Identifiers
PMID: 16000728
Source
Medline
License
Unknown

Abstract

The presence of a polar species of glycopeptidolipid (GPL) in carbon-starved Mycobacterium smegmatis has been reported previously. In this study, the complete structure of this GPL is established with the help of MALDI-TOF (matrix assisted laser desorption/ionization time of flight) and ESI (electrospray ionization) -MS, (13)C-SEFT (spin echo Fourier transform) -NMR spectroscopy, and HPLC analysis. In the molecule, two units of a 3,4-di-O-methyl derivative of rhamnose are attached to l-alaninol via a 1-->2 linkage. Various methyl derivatives of rhamnose and 6-deoxytalose were synthesized as standards to establish this structure. The accumulation of this polar GPL in M. smegmatis is sigB dependent, as a SigB-overproducing strain of M. smegmatis shows the presence of this spot in the exponential phase, and a sigB-knockout strain of M. smegmatis does not show the presence of any polar GPLs.

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