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Hydroxytyrosol encapsulated in biocompatible water-in-oil microemulsions: How the structure affects in vitro absorption.

Authors
  • Mitsou, Evgenia1
  • Dupin, Adeline2
  • Sassi, Abdessattar Hadj2
  • Monteil, Julien2
  • Sotiroudis, George T3
  • Leal-Calderon, Fernando2
  • Xenakis, Aristotelis4
  • 1 Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vassileos Constantinou Ave., 11635, Athens, Greece; Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110, Ioannina, Greece. , (Greece)
  • 2 Laboratoire Chimie et Biologie des Membranes et des Nanoobjets, Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, Pessac, France. , (France)
  • 3 Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vassileos Constantinou Ave., 11635, Athens, Greece. , (Greece)
  • 4 Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vassileos Constantinou Ave., 11635, Athens, Greece. Electronic address: [email protected] , (Greece)
Type
Published Article
Journal
Colloids and surfaces. B, Biointerfaces
Publication Date
Dec 01, 2019
Volume
184
Pages
110482–110482
Identifiers
DOI: 10.1016/j.colsurfb.2019.110482
PMID: 31539752
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Over the last years, the incorporation of natural antioxidants in food and pharmaceutical formulations has gained attention, delaying or preventing oxidation phenomena in the final products. In order to take full advantage of their properties, protection in special microenvironments is of great importance. The unique features of the natural phenolic compound hydroxytyrosol (HT) - including antioxidant, anti-inflammatory, antiproliferative and cardioprotective properties - have been studied to clarify its mechanism of action. In the present study novel biocompatible water-in-oil (W/O) microemulsions were developed as hosts for HT and subsequently examined for their absorption profile following their oral uptake. The absorption of HT in solution was compared with the encapsulated one in vitro, using a coculture model (Caco-2/TC7 and HT29-MTX cell lines). The systems were structurally characterized by means of Dynamic Light Scattering (DLS) and Electron Paramagnetic Resonance (EPR) techniques. The diameter of the micelles remained unaltered after the incorporation of 678 ppm of HT but the interfacial properties were slightly affected, indicating the involvement of the HT molecules in the surfactant monolayer. EPR was used towards a lipophilic stable free radial, namely galvinoxyl, indicating a high scavenging activity of the systems and encapsulated HT. Finally, after the biocompatibility study of the microemulsions the intestinal absorption of the encapsulated HT was compared with its aqueous solution in vitro. The higher the surfactants' concentration in the system the lower the HT concentration that penetrated the constructed epithelium, indicating the involvement of the amphiphiles in the antioxidant's absorption and its entrapment in the mucus layer. Copyright © 2019 Elsevier B.V. All rights reserved.

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