Affordable Access

Such hydrophobic peptides as dansylated mastoparan can elevate the fertilization membrane of sea urchin eggs.

Authors
  • Saito, K
  • Imoto, M
  • Nagano, N
  • Toriyama, M
  • Nakajima, T
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Oct 24, 1995
Volume
215
Issue
3
Pages
828–834
Identifiers
PMID: 7488048
Source
Medline
License
Unknown

Abstract

Melittin is known to be a major hydrophobic peptide component in honeybee venom that can cause as much elevation of fertilization membrane of sea urchin eggs as normal fertilization. The action of melittin has been thought to be closely related with its ability to facilitate the phospholipase A2 activity on the eggs. However, another peptide "mastoparan" from wasp venom was not found here to cause any elevation of the membrane, although it can activate the enzyme as well as melittin. On the other hand, mastoparan was found to get the membrane-elevating activity only when its amino groups were modified with hydrophobic substituents. N epsilon-Substituted mastoparan with a dansyl group in Lys11 residue was most effective among the analogs examined here. Our findings indicate that the facilitation of phospholipase by the peptides have little relation with the membrane generation. Such hydrophobic moiety as the dansyl group in the peptides must cause the cortical reaction on the eggs in cooperation with peptide moiety. The dansylated peptide will be a useful tool to induce the artificial fertilization of sea urchin eggs.

Report this publication

Statistics

Seen <100 times