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A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus

  • Li, Hao
  • Yang, Yanli
  • Zhang, Yan
  • Zhang, Songping
  • Zhao, Qizu
  • Zhu, Yuanyuan
  • Zou, Xingqi
  • Yu, Mengran
  • Ma, Guanghui
  • Su, Zhiguo
Publication Date
Sep 01, 2015
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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A purification scheme based on hydrophobic interaction chromatography was developed to separate inactivated foot-and-mouth disease virus (FMDV) from crude supernatant. About 92% recovery and 8.8-fold purification were achieved on Butyl Sepharose 4FF. Further purification on Superdex 200 resulted in another 29-fold purification, with 92% recovery. The columns were coupled through an intermediate ultrafiltration unit to concentrate the virus. The entire process was completed in about 3.5 h, with 75% final FMDV recovery, and 247-fold purification. The final product had purity above 98%, with over 99.5% of host cell DNA removed. High-performance size exclusion chromatography (HPSEC), Western blot, dynamic light scattering (DLS), and transmission electron microscopy (TEM) indicated that the purified virus contained the required antigen, and was structurally intact with a spherical shape and a particle size of 28 nm. (C) 2015 Elsevier Inc. All rights reserved.

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