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[HUVECs extraction and UHPLC LTQ Orbitrap analysis for screening vascular endothelium protective components in Kudiezi injection].

Authors
  • Liu, Si-Yi1, 2
  • Zhang, Hong-Zhu3
  • Fan, Jing4
  • Shang, Zhan-Peng1
  • Wang, Fei1
  • Li, Wei-Jie1
  • Liu, Ying1
  • Lu, Jian-Qiu1
  • Zhang, Jia-Yu1
  • 1 Beijing University of Chinese Medicine, Beijing 100029, China. , (China)
  • 2 Center for Drug Evaluation, China Food and Drug Administration, Beijing 100038, China. , (China)
  • 3 The First Hospital of Fangshan District, Beijing 102400, China. , (China)
  • 4 WuXi AppTec, Shanghai 200131, China. , (China)
Type
Published Article
Journal
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Publication Date
Sep 01, 2017
Volume
42
Issue
17
Pages
3362–3367
Identifiers
DOI: 10.19540/j.cnki.cjcmm.20170801.001
PMID: 29192447
Source
Medline
Keywords
License
Unknown

Abstract

An effective method has been employed as a tool for screening active components in Kudiezi injection by using cell chromatography and sensitive UHPLC-HR-MSn method. The potential bioactive components in Kudiezi injection could be selectively bound to the HUVECs target cells first. After cell target desensitization and inactivation, the chemical constituents with cell target affinity were identified by LC-MS, so as to screen the possible active components in Kudiezi injection. Based on the accurate mass measurements and the retention time, in total, 9 compounds were tentatively identified and characterized, including 4 sesquiterpene lactones, 3 phenolic acids and 2 flavonoids. HUVECs biospecific extraction coupled with UHPLC-LTQ-Orbitrap analysis could provide a rapid and efficient method for the identification of potential bioactive components in Kudiezi injection, and provide the reference for further research on its effective materials basis.

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