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Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice.

Authors
  • Witonsky, Sharon G
  • Gogal, Robert M Jr
  • Duncan, Robert B Jr
  • Norton, Heather
  • Ward, Dan
  • Yang, Jibing
  • Lindsay, David S
Type
Published Article
Journal
The Journal of parasitology
Publication Date
Aug 01, 2005
Volume
91
Issue
4
Pages
830–837
Identifiers
PMID: 17089750
Source
Medline
License
Unknown

Abstract

Immunodeficient B-cell-deficient mice (mmuMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RB(high)) and memory (CD44(high)) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.

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