Affordable Access

Access to the full text

Human tRNA genes function as chromatin insulators.

Authors
  • Jr, Raab
  • J, Chiu
  • J, Zhu
  • S, Katzman
  • S, Kurukuti
  • Pa, Wade
  • David Haussler
  • Rt, Kamakaka
Type
Published Article
Journal
The EMBO Journal
Publisher
EMBO
Publication Date
Nov 26, 2011
Volume
31
Issue
2
Pages
330–330
Identifiers
DOI: 10.1038/emboj.2011.406
PMID: 22085927
PMCID: PMC3261562
Source
UCSC Bioengineering biomedical-ucsc
License
Green

Abstract

Insulators help separate active chromatin domains from silenced ones. In yeast, gene promoters act as insulators to block the spread of Sir and HP1 mediated silencing while in metazoans most insulators are multipartite autonomous entities. tDNAs are repetitive sequences dispersed throughout the human genome and we now show that some of these tDNAs can function as insulators in human cells. Using computational methods, we identified putative human tDNA insulators. Using silencer blocking, transgene protection and repressor blocking assays we show that some of these tDNA-containing fragments can function as barrier insulators in human cells. We find that these elements also have the ability to block enhancers from activating RNA pol II transcribed promoters. Characterization of a putative tDNA insulator in human cells reveals that the site possesses chromatin signatures similar to those observed at other better-characterized eukaryotic insulators. Enhanced 4C analysis demonstrates that the tDNA insulator makes long-range chromatin contacts with other tDNAs and ETC sites but not with intervening or flanking RNA pol II transcribed genes.

Report this publication

Statistics

Seen <100 times