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[A human recombinant erythropoietin produced in human lymphoblastoid cells].

Authors
  • Bounemra-Ben Ghanem, A
  • Cherif, W
  • Lambin, P
  • Jenhani, F
  • Boukef, K
Type
Published Article
Journal
Journal de pharmacie de Belgique
Publication Date
Jan 01, 1997
Volume
52
Issue
5
Pages
177–180
Identifiers
PMID: 9432525
Source
Medline
License
Unknown

Abstract

A human recombinant erythropoietin is produced by transfection of a human EBV immortalised lymphoblastoid cell line by human erythropoietin gene. This cell line was selected because it is eucaryotic, human, able to grow in suspension and neither clonogenic nor tumorigenic. Purification of erythropoietin from culture supernatants of lymphoblastoid cells was described and structural, biochemical and functional characteristics were investigated in several studies. Results obtained for this kind of recombinant erythropoietin were similar to those obtained regarding the protein expressed in CHO cells suggesting that both molecules have the same efficiency in clinical applications.

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